Quantification of Bacterial Indicators and Zoonotic Pathogens in Dairy Wastewater Ponds

Quantification of bacterial indicators and zoonotic pathogens in dairy wastewater ponds.

Zoonotic pathogens in land-applied dairy wastewaters are a potential health risk. The occurrence and abundance of 10 pathogens and 3 fecal indicators were determined by quantitative real-time PCR (qPCR) in samples from 30 dairy wastewaters from southern Idaho. Samples tested positive for Campylobacter jejuni, stx(1)- and eaeA-positive Escherichia coli, Listeria monocytogenes, Mycobacterium aviu...

The characterization of microorganisms in dairy wastewater storage ponds.

Dairy wastewaters from storage ponds are commonly land applied to irrigate forage crops. Given that diverse microbial populations are associated with cattle feces, the objective of this study was to use a culture-independent approach to characterize bacteria and archaea in dairy wastewaters. Using domain-specific primers, a region of the 16S rRNA gene was amplified from pooled DNA extracts from...

Fecal Indicators and Zoonotic Pathogens in Household Drinking

Quantification of Zoonotic Bacterial Pathogens within Commercial Poultry Processing Water Samples Using Droplet Digital PCR

Raw poultry and poultry products are a significant source of zoonotic bacterial pathogen transmission; thus the sensitive detection of major zoonotic pathogens (Salmonella spp., Campylobacter jejuni, and Listeria monocytogenes) is a vital food safety issue. Recently, third generation PCR technology, known as droplet digital PCR (ddPCR) has been developed to be more accurate and sensitive to det...

Performance evaluation of wastewater stabilization ponds in Yazd-Iran

Background: Yazd waste stabilization pond facilities consist of three stabilization pond systems, module 1, module 2 and module 3 that AWSP module 1 has started its operation. The existing facilities have had several problems in their operation. The objectives of this research were to evaluate the performance of stabilization ponds in wastewater treatment of the city of Yazd, due to several pro...